Sequencing

The Human Genome Project was completed in ten years and cost three billion dollars. Due to recent advances in high-throughput sequencing technology, an entire human genome can now be sequenced in a few days for one millionth of the original cost. The Sequencing Core Technology Platform is used by researchers in genomics and systems biology to rapidly collect large amounts of data on DNA and RNA sequences from humans, vertebrate and invertebrate animals, plants, and microbes in order to characterize their genomes (DNA), transcriptomes (RNA), and genetic diversity in populations (sequence variants).

Applications include the analysis of genomes, gene expression patterns, regulatory interactions, epigenetics, and how sequences vary within populations and between species. Microbes: Applications include the analysis of genomes, gene expression patterns, regulatory interactions, epigenetics, and how sequences vary within populations and between species. Other applications include investigating interactions between proteins and nucleic acids (e.g., using ChIP-seq or PAR-CLIP) and analyzing samples collected from the environment (metagenomics).

Data analysis for the Sequencing CTP is supported by NYUAD-CGSB’s Bioinformatics Core. For further information on resources for collection and analysis of sequencing data, visit the NYU Genomics Core website.

Equipment List

  • Agilent Bioanlyzer 2100 Electrophoresis & Flow Cytometry
  • ECO Real-time PCR
  • HiSeq 2500
  • MiSeq
  • Ion PGM Sequencer
  • Ion Proton Sequencer
  • StepOnePlus Real-Time PCR System
  • NeoPrep