Molecular and Cell Biology

The Molecular and Cell Biology platform aids in the cloning and subcloning of genes into different vectors; the creation of proteins that are used in biotechnology research; and the biophysical and biochemical structural characterization of different macromolecules.

Gene Cloning and Manipulation
Cloning and subcloning of genes into a variety of different vectors; gene manipulation that alters specific domains, or simply changes fusion tags on proteins; mutagenesis, which alters single amino acids.

Protein Expression and Purification
Includes an efficient E. coli expression system to express verity of protein samples (including human proteins) with purity higher than 95%.

Biophysical and Biochemical Structural Characterization of Different Macromolecules
Characterizes the structure of different macromolecules and determines the thermal and chemical stability of biological macromolecules; analyzes the energetics of binding different molecules to a biological sample; screens for binding partners to the macromolecule using small molecule libraries as the first method in drug discovery and design.

Genomics at single-cell resolution
The Fluidigm system is an integrative microfluidic-based system that enables several complementary genomic applications. The system provides mid- to high-throughput gene expression, genotyping, digital PCR, single-cell expression profiling, and sample preparation for single-cell whole-genome or targeted sequencing. The system has several components accommodating each of these applications at various throughput scales. The system allows running complex processes that would normally be unfeasible using standard approaches.

Equipment List

  • Active 4 Science Shell Ground Model for use on RPM
  • AerosolSense tm Sampler Instrument
  • AMSCO® 430LS Medium Steam Sterilizer
  • Arpege40 Cryotanks
  • Autoclave - Hirayama Hiclave HV-110
  • Autoclave - Rodwell Sapphire 660
  • Automated Microscope - Biotek LionHeart FX
  • Autoscratch-S
  • Bioreactor system-Epiphyte3 LEX-48
  • Centrifuge - Beckman Coulter Allegra X12
  • Centrifuge - Beckman Coulter Avanti J26XPI
  • Chemagic 360-D
  • ChemiDoc MP Imaging System 
  • Circular Dichroism Spectrometer - Applied Photophysics Chirascan plus
  • Differential Scanning Calorimetry - TA instruments Nano DSC
  • Endoscopy System (Small Animal)
  • Flow Cytometer - ThermoFisher Attune NxT
  • Flow Cytometer-CYTEK- AURORA
  • FPLC - GE Lifesciences AKTAexplorer
  • HighThroughput qPCR - Fluidigm Biomark HD
  • Isothermal Calorimetry - TA instruments Nano ITC
  •  Metabolic Analyzer-Agilent Seahorse XFe96
  • Microcalorimeter - TA Instruments TAM IV nano
  • Microplate reader - Biotek Cytation 5
  • Mini Protean Tetra Cell & Trans-Blot Electrophoresis Kits
  • Nanometers
  • qPCR- Applied Biosystems QS5
  • Qubit 2.0 Fluorometer
  • Random Positioning Machine RPM2.0
  • Refeyn TwoMP Mass Photometer
  • Rheometer - Rheolution Elastosens Bio2
  • Spectrophotometer Nanodrop 1 sample
  • Spectrophotometer Nanodrop 8 samples
  • Super Speed Centrifuge - Thermo Scientific Lynx 6000
  • Tube Luminometer-Lumat 3
  • Ultracentrifuge - Beckman Coulter XPN 90
  • Uvitec Transilluminator
  • UV-VIS Spectrophotometer - Shimadzu UV-2700

Tissue Culture 

  • Tissue Culture 1 to 7
  • BSL-2 Pathogenic Bacterial lab