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Molecular and Cell Biology

The Molecular and Cell Biology Core Technology Platform provides a broad range of services and expertise that aid scientists in performing research at the molecular, single cell, and tissue culture level. Research activities carried out at NYUAD in this core include:

Gene Editing and Molecular Cloning:
● Genome editing services for targeted gene knockout, knock-in, and gene modifications
● Molecular cloning techniques for gene insertion, deletion, and manipulation
● Recombinant DNA technology for constructing expression vectors and genetic engineering

Protein Expression and Purification:
● Recombinant protein expression in bacterial, yeast, insect, or mammalian cell
● Protein purification using chromatography techniques such as affinity chromatography, ion exchange chromatography, and size exclusion chromatography
● Characterization of protein structure, function, and interactions

Cell Culture and Functional Assays:
● Cell culture services for maintaining and propagating cell lines, primary cells, and stem cells
● Functional assays to study cell proliferation, viability, apoptosis, migration, and differentiation
● High-content screening (HCS) and automated microscopy (Brightfield and Fluorescence) for analyzing cellular phenotypes and responses
● Live-cell imaging to track dynamic processes and monitor cellular behavior in real-time

Flow Cytometry and Cell Sorting:
● Flow cytometry analysis for quantifying and characterizing cells based on surface markers, intracellular proteins, and DNA content
● Fluorescence-activated cell sorting (FACS) for isolating specific cell populations based on their phenotypic properties

Biochemical Assays and Enzymology:
● Enzyme assays to measure enzyme activity, substrate specificity, and kinetics
● Biochemical analysis of proteins, nucleic acids, lipids, and metabolites
● Assay development and optimization for drug discovery, biomarker identification, and pathway analysis

Equipment List

  • Active 4 Science Shell Ground Model for use on RPM
  • Analytical Ultracentrifuge-Optima AUC- Beckman Coulter
  • AerosolSense tm Sampler Instrument
  • AMSCO® 430LS Medium Steam Sterilizer
  • Arpege40 Cryotanks
  • Autoclave - Hirayama Hiclave HV-110
  • Autoclave - Rodwell Sapphire 660
  • Automated Microscope - Biotek LionHeart FX
  • Autoscratch-S
  • Bioreactor system-Epiphyte3 LEX-48
  • Biostat® B-DCU Bioreactor
  • Centrifuge - Beckman Coulter Allegra X12
  • Centrifuge - Beckman Coulter Avanti J26XPI
  • Chemagic 360-D
  • ChemiDoc MP Imaging System 
  • Circular Dichroism Spectrometer - Applied Photophysics Chirascan plus
  • Differential Scanning Calorimetry - TA instruments Nano DSC
  • Endoscopy System (Small Animal)
  • Flow Cytometer - ThermoFisher Attune NxT
  • Flow Cytometer-CYTEK- AURORA
  • FPLC - GE Lifesciences AKTAexplorer
  • HighThroughput qPCR - Fluidigm Biomark HD
  • Isothermal Calorimetry - TA instruments Nano ITC
  •  Metabolic Analyzer-Agilent Seahorse XFe96
  • Microcalorimeter - TA Instruments TAM IV nano
  • Microplate reader - Biotek Cytation 5
  • Mini Protean Tetra Cell & Trans-Blot Electrophoresis Kits
  • NanoDrop One/OneC UV-Vis Spectrophotometer
  • Nanometers
  • qPCR- Applied Biosystems QS5
  • Qubit 2.0 Fluorometer
  • Random Positioning Machine RPM2.0
  • Refeyn TwoMP Mass Photometer
  • Rheometer - Rheolution Elastosens Bio2
  • Spectrophotometer Nanodrop 1 sample
  • Spectrophotometer Nanodrop 8 samples
  • Super Speed Centrifuge - Thermo Scientific Lynx 6000
  • Tube Luminometer-Lumat 3
  • Ultracentrifuge - Beckman Coulter XPN 90
  • Uvitec Transilluminator
  • UV-VIS Spectrophotometer - Shimadzu UV-2700

Tissue Culture 

  • Tissue Culture 1 to 7
  • BSL-2 Pathogenic Bacterial lab
  • Biorad Zoe Imager