The Molecular and Cell Biology platform aids in the cloning and subcloning of genes into different vectors; the creation of proteins that are used in biotechnology research; and the biophysical and biochemical structural characterization of different macromolecules.
Gene Cloning and Manipulation
Cloning and subcloning of genes into a variety of different vectors; gene manipulation that alters specific domains, or simply changes fusion tags on proteins; mutagenesis, which alters single amino acids.
Protein Expression and Purification
Includes an efficient E. coli expression system to express verity of protein samples (including human proteins) with purity higher than 95%.
Biophysical and Biochemical Structural Characterization of Different Macromolecules
Characterizes the structure of different macromolecules and determines the thermal and chemical stability of biological macromolecules; analyzes the energetics of binding different molecules to a biological sample; screens for binding partners to the macromolecule using small molecule libraries as the first method in drug discovery and design.
Genomics at single-cell resolution
The Fluidigm system is an integrative microfluidic-based system that enables several complementary genomic applications. The system provides mid- to high-throughput gene expression, genotyping, digital PCR, single-cell expression profiling, and sample preparation for single-cell whole-genome or targeted sequencing. The system has several components accommodating each of these applications at various throughput scales. The system allows running complex processes that would normally be unfeasible using standard approaches.
Equipment List
Tissue Culture
