Molecular and Cell Biology

The Molecular and Cell Biology platform aids in the cloning and subcloning of genes into different vectors; the creation of proteins that are used in biotechnology research; and the biophysical and biochemical structural characterization of different macromolecules.

Gene Cloning and Manipulation
Cloning and subcloning of genes into a variety of different vectors; gene manipulation that alters specific domains, or simply changes fusion tags on proteins; mutagenesis, which alters single amino acids.

Protein Expression and Purification
Includes an efficient E. coli expression system to express verity of protein samples (including human proteins) with purity higher than 95%.

Biophysical and Biochemical Structural Characterization of Different Macromolecules
Characterizes the structure of different macromolecules and determines the thermal and chemical stability of biological macromolecules; analyzes the energetics of binding different molecules to a biological sample; screens for binding partners to the macromolecule using small molecule libraries as the first method in drug discovery and design.

Genomics at single-cell resolution
The Fluidigm system is an integrative microfluidic-based system that enables several complementary genomic applications. The system provides mid- to high-throughput gene expression, genotyping, digital PCR, single-cell expression profiling, and sample preparation for single-cell whole-genome or targeted sequencing. The system has several components accommodating each of these applications at various throughput scales. The system allows running complex processes that would normally be unfeasible using standard approaches.

  • AKTA pure 25 M 
  • Arpege40 Cryotanks
  • Autoclave - Hirayama Hiclave HV-110 (First Floor)
  • Autoclave - Hirayama Hiclave HV-110 (Ground Floor)
  • Autoclave - Rodwell Sapphire 660 (First Floor)
  • Autoclave - Rodwell Sapphire 660 (Ground Floor)
  • Autoclave-Hiclave HV-110
  • Automated Microscope - Biotek LionHeart FX
  • Binder CO2 Incubator
  • Biorad Zoe Imager
  • Bioreactor system-Epiphyte3 LEX-48 
  • Centrifuge - Beckman Coulter Allegra X12
  • Centrifuge - Beckman Coulter Avanti J26XPI
  • Chemagic 360-D
  • ChemiDoc MP Imaging System
  • Circular Dichroism Spectrometer - Applied Photophysics Chirascan plus
  • Class II Type A2 biological safety cabinets 
  • Classic Prestige Medical Autoclaves
  • Countess II cell counter
  • Differential Scanning Calorimetry - TA instruments Nano DSC
  • EVOS Floid Imaging System
  • Flow Cytometer - BD Biosciences Accuri C6
  • Flow Cytometer - ThermoFisher Attune NxT
  • Flow Cytometer, Cytek Aurora
  • Fluidigm Biomark HD
  • HighThroughput qPCR - Fluidigm Biomark HD
  • Isothermal Calorimetry - TA instruments Nano ITC
  • Mastercycler Pro PCR
  • Mediaclave 10 & Mediajet Vario 
  • Metabolic Analyzer-Agilent Seahorse XFe96
  • Microcalorimeter - TA Instruments TAM IV nano
  • Microfuge 22R Beckman Coulter
  • Microplate reader - Biotek Cytation 5
  • Microplate Reader - Biotek Synergy H1
  • Mini Horizontal Electrophoresis i-MyRun Units
  • Mini Protean Tetra Cell & Trans-Blot Electrophoresis Kits
  • Multigene Optimax PCR
  • Multi-Mode Dispenser -Biotek MultiFlo FX 
  • Nanometers
  • Panasonic Heated Incubator
  • Pro250 Cell Homogenizer
  • QPCR - Agilent Technologies Stratagene Mx3005P
  • qPCR- Applied Biosystems QS5
  • Qubit 2.0 Fluorometer
  • Rheometer - Rheolution Elastosens Bio2
  • Shaker - New Brunswick Scientific Excella E24
  • Shaker - New Brunswick Scientific I26
  • Shaker - New Brunswick Scientific Innova 44
  • Sonics Vibra Cell Sonicators (VCXX130, VCX750)
  • Stopped-Flow Reaction Analyzer - Applied Photophysics SX20
  • Super Speed Centrifuge - Thermo Scientific Lynx 6000
  • Tube Luminometer-Lumat 3
  • Ultracentrifuge - Beckman Coulter XPN 90
  • Uvitec Fire Reader Gel Documentation System
  • Uvitec Transilluminator
  • UV-VIS Spectrophotometer - Shimadzu UV-2700

Tissue Culture 

  • Tissue Culture 1 to 5
  • BSL-2 Pathogenic Bacterial lab
  • Biorad Zoe Imager
  • Covid Screening Lab