Sequencing
The sequencing facility enables researchers to rapidly collect large amounts of data on DNA and RNA sequences. Researchers are using the latest Illumina and Oxford Nanopore sequencing technology to sequence samples from humans, model organisms, and non-model organisms to characterize their genomes (DNA), transcriptomes (RNA), and genetic diversity in populations (sequence variants). In addition, the 10X Genomics platform facilitates various downstream analysis at single-cell resolution in fields such as developmental biology, cancer research, and immunology. Other applications include investigating interactions between proteins and nucleic acids (e.g., using ChIP-seq or PAR-CLIP) and analyzing samples collected from the environment (metagenomics).
Upon reception of DNA or RNA samples, a team of highly skilled specialists will proceed with library preparation either manually or with automation solutions and ensure the highest quality and the best reproducibility rates are met. Sequencing data will then be processed on the HPC and results delivered to the researchers.
Equipment List
- 10X Genomics - Chromium Controller
- Agilent 4200 TapeStation
- Agilent Bioanalyzer 2100
- Automated Liquid Handling - BMS Myra
- Illumina MiSeq
- Illumina NextSeq 550
- Illumina NovaSeq 6000
- Juno System
- MIC 4- channel -qPCR system
- Oxford Nanopore Technologies MinION Mk1C
- Oxford Nanopore Technologies PromethION 2 Integrated (P2i)
- Real-Time PCR - ThermoFisher SteponePlus
- SeqStudio Genetic Analyzer