Spectrometry and Spectroscopy

The Spectrometry and Spectroscopy Core Technology Platform assists researchers in the following applications:

Chemical Discovery, Synthesis, and Characterization

This facility maintains and operates a wide range of tools for separation and characterization of compounds which aids in the synthesis and discovery of chemical compounds. This core facility has a general use Q-TOF and 500 MHz NMR that synthetic chemists use for compound characterization and identification. This facility is equipped with an FT-IR, UV-VIS-NIR, and fluorescence for photophysical analysis and HPLCs to aid in precision separation. The platform offers a broad array of equipment that facilitates the synthesis and isolation of chemical compounds. Furthermore, this core is equipped with a higher field 600 MHz NMR including a cryoprobe and the first wide bore solid state 600 MHz NMR spectrometer.

Metabolomics

Metabolomics is a term used for the characterizing and understanding of all metabolites present in living organisms. The metabolome can help us understand biochemical processes, diseases, and biodiversity by using spectroscopic and spectrometric techniques to characterize and quantify complex biological mixtures. The primary piece of equipment for this purpose is the Agilent Q-TOF 6548, but the facility is in the process of expanding the mass spectrometry facilities to better serve our research applications.

Protein Structure

As proteins are large molecules, the same spectroscopic techniques can be used to characterize and validate these compounds, only with additional time, care, and data handling. The 600 MHz NMR with cryoprobe is mostly dedicated to understand the dynamic structure proteins in solutions.

Equipment List

  • 500 MHz NMR Spectrometer - Bruker 500 MHz Avance
  • 600 MHz NMR Spectrometer - Bruker 600 MHz Ascend UHD
  • 600 MHz Wide Bore NMR Spectrometer - Bruker 600 MHz Aeon
  • Accelerated Solvent Extractor -Thermofisher Dionex ASE 350
  • Centrifuge-thermofisher Savant SCA210A
  • Certus Flex Liquid Dispenser
  • Chemspeed
  • EPR/ESR Spectrometer - Bruker EMXnano
  • EVOQ Tandem Quad LC-MS
  • Fluorescence Spectrometer - Perkin Elmer LS 55
  • Fluoromax 4: Spectrofluorometer
  • Fourier Transfom Infrared Spectrometer - Agilent FT-IR 670/630
  • Gas Chromatography Mass Spectrometer - Perkin Elmer Clarus 610
  • Glovebox - Innovative Technology PL-HE-2GB
  • HPLC (Analytical Scale) - Agilent 1260
  • HPLC (Semi-prep) - Agilent 1260
  • LC-MSMS - Agilent 6538
  • LC-MSMS - Bruker Impact II
  • Liquid Chromatography/MALDI Mass Spectrometry (LC/MALDI-MSMS) - Bruker 7T solariX2xR
  • Lypholizer - Christ Alpha 1-2 LD plus
  • MCP 5100 Modular Circular Polarimeter
  • Microwave Reactor - CEM Discover
  • SEAL AutoAnalyzer 3 HR
  • Thermo EASY-nLC Q-Exactive HF Orbitrap MS
  • Thermo System Base Vanquish - Analytical grade
  • Ultraviolet-Vis Spectrophometer - Perkin Elmer Lambda 25
  • Ultraviolet-visible-nearinfrared Spectrophotometer - Agilent Cary 5000