While our physiological development resulted in permanent lymph nodes, tertiary lymphoid organs are temporary in nature and develops in response to chronic inflammation and autoimmune diseases. Both lymph nodes and tertiary lymphoid organs are vital in disease resolution and disease progression. Tertiary lymphoid organs and lymph nodes have cellular players, anatomical organization, chemokine pool, and vascular similarities and our lab aims to use our growing knowledge on immunomechanobiology and bioengineering techniques to recapitulate such organs in vitro for mechanistic studies, as well as translational applications.