Molecular and Cell Biology
The Molecular and Cell Biology Core Technology Platform provides a broad range of services and expertise that aid scientists in performing research at the molecular, single-cell, and tissue culture level. Research activities carried out at NYUAD in this core include:
Editing and Molecular Cloning
- Genome editing services for targeted gene knockout, knock-in, and gene modifications
- Molecular cloning techniques for gene insertion, deletion, and manipulation
- Recombinant DNA technology for constructing expression vectors and genetic engineering
Protein Expression and Purification
- Recombinant protein expression in bacterial, yeast, insect, or mammalian cell
- Protein purification using chromatography techniques such as affinity chromatography, ion exchange chromatography, and size exclusion chromatography
- Characterization of protein structure, function, and interactions
Cell Culture and Functional Assays
- Cell culture services for maintaining and propagating cell lines, primary cells, and stem cells
- Functional assays to study cell proliferation, viability, apoptosis, migration, and differentiation
- High-content screening (HCS) and automated microscopy (Brightfield and Fluorescence) for analyzing cellular phenotypes and responses
- Live-cell imaging to track dynamic processes and monitor cellular behavior in real-time
Flow Cytometry and Cell Sorting
- Flow cytometry analysis for quantifying and characterizing cells based on surface markers, intracellular proteins, and DNA content
- Fluorescence-activated cell sorting (FACS) for isolating specific cell populations based on their phenotypic properties
Biochemical Assays and Enzymology
- Enzyme assays to measure enzyme activity, substrate specificity, and kinetics
- Biochemical analysis of proteins, nucleic acids, lipids, and metabolites
- Assay development and optimization for drug discovery, biomarker identification, and pathway analysis
Equipment List
- Active 4 Science Shell Ground Model for use on RPM
- Analytical Ultracentrifuge-Optima AUC- Beckman Coulter
- AerosolSense tm Sampler Instrument
- AMSCO® 430LS Medium Steam Sterilizer
- Arpege40 Cryotanks
- Autoclave - Hirayama Hiclave HV-110
- Autoclave - Rodwell Sapphire 660
- Automated Microscope - Biotek LionHeart FX
- Autoscratch-S
- Bioreactor system-Epiphyte3 LEX-48
- Biostat® B-DCU Bioreactor
- Centrifuge - Beckman Coulter Allegra X12
- Centrifuge - Beckman Coulter Avanti J26XPI
- Chemagic 360-D
- ChemiDoc MP Imaging System
- Circular Dichroism Spectrometer - Applied Photophysics Chirascan plus
- Differential Scanning Calorimetry - TA instruments Nano DSC
- Endoscopy System (Small Animal)
- Flow Cytometer - ThermoFisher Attune NxT
- Flow Cytometer-CYTEK- AURORA
- FPLC - GE Lifesciences AKTAexplorer
- HighThroughput qPCR - Fluidigm Biomark HD
- Isothermal Calorimetry - TA instruments Nano ITC
- Metabolic Analyzer-Agilent Seahorse XFe96
- Microcalorimeter - TA Instruments TAM IV nano
- Microplate reader - Biotek Cytation 5
- Mini Protean Tetra Cell & Trans-Blot Electrophoresis Kits
- NanoDrop One/OneC UV-Vis Spectrophotometer
- Nanometers
- qPCR- Applied Biosystems QS5
- Qubit 2.0 Fluorometer
- Random Positioning Machine RPM2.0
- Refeyn TwoMP Mass Photometer
- Rheometer - Rheolution Elastosens Bio2
- Spectrophotometer Nanodrop 1 sample
- Spectrophotometer Nanodrop 8 samples
- Super Speed Centrifuge - Thermo Scientific Lynx 6000
- Tube Luminometer-Lumat 3
- Ultracentrifuge - Beckman Coulter XPN 90
- Uvitec Transilluminator
- UV-VIS Spectrophotometer - Shimadzu UV-2700
Tissue Culture
- Tissue Culture 1 to 7
- BSL-2 Pathogenic Bacterial lab
- Biorad Zoe Imager